"Both processes employ antibodies to specific antigens (e.g. cellular proteins like GFAP or EMA). There are now a significant number of antibodies, and typically multiple antibodies (a panel) will be used based on the differential diagnosis suggested by the patient's history and standard H&E examination of the sample."
"Both processes employ antibodies to specific antigens (e.g. cellular proteins like GFAP or EMA). There are now a significant number of antibodies, and typically multiple antibodies (a panel) will be used based on the differential diagnosis suggested by the patient's history and standard H&E examination of the sample."
Expected headings
"Direct vs indirect"
"Slide preparation"
"Antigen retrieval"
"Primary antibody"
"Secondary antibody"
"Chromogen application"
"Counterstaining"
"Internal control"
"There are many variations on exactly how immunohistochemistry is performed and it is outside the scope of this article to cover any in great detail. Still, broadly speaking they can be divided into:"
"Both processes employ antibodies to specific antigens (e.g. cellular proteins like GFAP or EMA). There are now a significant number of antibodies, and typically multiple antibodies (a panel) will be used based on the differential diagnosis suggested by the patient's history and standard H&E examination of the sample."
"immunofluorescence, where the antibody is tagged with a compound that fluoresces; and"