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Lint: valentino-syndrome

Emphasis
warning

"Treatment is a surgical emergency, with patients requiring repair of the rupture 1-3. Postoperatively, management should consider and address the cause of the peptic ulcer (e.g. Helicobacter pylori eradication therapy) 1-3."

Line 17:193 · Italics should be used only in exceptional circumstances: '<em>Helicobacter pylori</em>'
Oxford Comma
suggestion

"localised pain, tenderness and guarding over the right lower abdominal quadrant"

Line 7:18 · Use the Oxford comma in 'pain, tenderness and guarding'.
Commas
suggestion

"Radiographic investigations, such as ultrasound or CT, may be performed with acute appendicitis being the working diagnosis, and often reveal a normal or mildly inflamed appendix 1-5. However, patients may instead have radiographic features of a ruptured peptic ulcer, including subtle pneumoretroperitoneum, with accompanying free fluid and adjacent fat stranding demonstrable in the right paracolic gutter and around the appendix, reflecting the pathophysiological basis of the condition 2,4,5."

Line 15:220 · More than 5 commas in a single sentence might make it more difficult to read.