"Under indirect immunofluorescence (IIF) microscopy, four major ANA staining patterns are observed, due to the varying intranuclear distribution of antigenic targets 1:"
"a broad array of antigen specificities generates a speckled pattern, including those raised against U1-RNP, Sm, and La"
"antibodies raised against components of the PM/Scl and 7-2RNP complexes; U3RNP (fibrillarin); RNA polymerases; and Scl70 (topoisomerase I)"
"antibodies raised against components of the PM/Scl and 7-2RNP complexes; U3RNP (fibrillarin); RNA polymerases; and Scl70 (topoisomerase I)"
"ANAs are implicated in the pathophysiology of numerous diseases, including many inflammatory rheumatic diseases. The ANA IIF assay is highly sensitive but minimally specific; low-titre positive ANA results occurs in approximately 13.8% of the general population 2, though some studies report up to 40% prevalence 3. This fact lacks any well-described clinical consequences 4."
"ANAs are implicated in the pathophysiology of numerous diseases, including many inflammatory rheumatic diseases. The ANA IIF assay is highly sensitive but minimally specific; low-titre positive ANA results occurs in approximately 13.8% of the general population 2, though some studies report up to 40% prevalence 3. This fact lacks any well-described clinical consequences 4."
"Loose associations are often made between particular ANA staining patterns and likely underlying autoimmune disorders, but no pattern is completely specific for any single disease. Following a positive ANA result, solid phase assays may be used to detect specific disease-associated autoantibody subtypes to aid in diagnosis."
"Loose associations are often made between particular ANA staining patterns and likely underlying autoimmune disorders, but no pattern is completely specific for any single disease. Following a positive ANA result, solid phase assays may be used to detect specific disease-associated autoantibody subtypes to aid in diagnosis."
"Grouped by IIF staining pattern, diseases associated with positive ANA titre include 5:"
"U1-RNP"
"MCTD (95-100% in high titre with no other ANA subtype present)"
"SS-A (Ro)"
"SS-B (La)"
"homogenous"
"homogenous"
"Sm"
"Scl-70"
"Jo-1"
Expected headings
"Associations"
"antibodies raised against centromere proteins A, B and C"
"antibodies raised against components of the PM/Scl and 7-2RNP complexes; U3RNP (fibrillarin); RNA polymerases; and Scl70 (topoisomerase I)"
"antibodies raised against components of the PM/Scl and 7-2RNP complexes; U3RNP (fibrillarin); RNA polymerases; and Scl70 (topoisomerase I)"
"antibodies raised against components of the PM/Scl and 7-2RNP complexes; U3RNP (fibrillarin); RNA polymerases; and Scl70 (topoisomerase I)"
"ANAs are implicated in the pathophysiology of numerous diseases, including many inflammatory rheumatic diseases. The ANA IIF assay is highly sensitive but minimally specific; low-titre positive ANA results occurs in approximately 13.8% of the general population 2, though some studies report up to 40% prevalence 3. This fact lacks any well-described clinical consequences 4."
"PSS (15-43%); associated with more severe organ involvement (e.g. pulmonary fibrosis) 4"
"polymyositis (30%); particularly associated with interstitial lung disease 4"